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For binding assays, sufficient recombinant homodimeric, chimeric proteins containing a siglec domain and a Fc domain per subunitFor comparison of substrate specificity, an iceLogo software was used to generate substrate specificity logos for amino acids at positions adjacent to the identified cleavage sitesFor detection of AX-B-HSA adducts, blots had been incubated with HRP-streptavidin (one:one thousand dilution), and biotinpositive indicators have been detected with ECL
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For the yeast-based screening methodology described listed here to be productive in an inducible methodFor these analyses peptides that were found in a number of experiments were positioned into a solitary table and the relative levels of peptide in every of the experimental replicates were colour codedFor these factors, ECFCs are deemed accurate EPCs progeny with all the phenotypic and practical characteristics of endothelial cells linked to attributes of stem/progenitor cells
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