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Trophic enteral feeding was started within the first 24?h of life at 10?mL/kg per day in infants weighing Venetoclax cost of PN and full enteral feeding time. Postnatal growth retardation was defined as bodyweight Histone demethylase Bronchopulmonary dysplasia (BPD) was defined as the need for oxygen at 36 weeks of postmenstrual age. Necrotizing enterocolitis (NEC) was defined using Bell's classification as stage II or more.[14] Patent ductus arteriosus (PDA), diagnosed on echocardiography was considered clinically significant when it required medical treatment. Intraventricular hemorrhage (IVH) grade II or higher was defined using the Papile et?al. classification.[15] Cystic periventricular leukomalacia (cPVL) was diagnosed on cranial ultrasonography. Retinopathy of prematurity (ROP) was defined as threshold ROP or higher stages of ROP according to indirect ophthalmoscopy. Serum glucose, sodium, potassium, chloride, calcium, phosphorus, urea, creatinine, triglyceride, serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase and direct bilirubin were measured weekly. Serum IGF-I was measured using enzyme-linked immunosorbent assay (ELISA) kits (Biosource Human IGF-1 Ctyelisa; Biosource, Nivelles, France; sensitivity 4.9?ng/mL) and IGFBP-3 was measured using ELISA kits (Biosource Human IGFBP-3 Ctyelisa; Biosource; sensitivity 10.5?ng/mL) at 40 weeks of gestation. Serum thyroxine Enzalutamide in vitro and thyroid-stimulating hormone were measured on radioimmunoassay at 1 and 3 postnatal weeks of life. Student's t-test was used for normally distributed continuous variables. Categorical data were analyzed using the chi-squared test and Fisher's exact test. Differences between protein and caloric intakes in both groups were analyzed using ANOVA and t-test. Multivariate analysis was performed using logistic regression in order to adjust for the effects of the following contributing factors: birthweight, gestational age, antenatal steroids, days on mechanical ventilation and neonatal morbidity (PDA, BPD, IVH and cPVL). Results are expressed as adjusted odds ratios and 95% confidence intervals. The Wald test was used to test for statistical significance on multivariance analysis (P