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8% of all sequences were identified as chimeras and removed prior to re-sampling. Clustering at 5% nucleotide dissimilarity on 4,300 sequences per sample yielded 23,431 clusters of which 14,352 were singletons or doubletons. For the bacterial 16S rRNA genes, a total of 5,501,355 raw paired end reads produced from 70 samples yielded 4,130,058 assembled and quality-filtered reads. A total of 1.3% of filtered reads were identified as chimeras and removed. Clustering at 3% nucleotide dissimilarity on 23,000 sequences per sample yielded 18,355 OTUs of which 4736 were singletons and 1840 were doubletons. Community Differences with Sample Size and Extraction Method For both the bacterial and fungal communities, Margalef��s richness (d, ANOVA, 28S: F = 11.25, P check details (H��, 28S: F = 9.1, P Azastene individuals (N, 28S: F = 7.9, P AZD5363 terms of size �� replicate (28S: F = 0.86, P > 0.10, 16S: F = 0.94, P > 0.10). Permutational dispersion (PERMDISP) revealed significant overall differences in sample dispersion among extraction sizes (28S: F = 43.6, P = 0.001, 16S: F = 14.47, P = 0.001) with dispersion values decreasing with increasing sample extraction size (28S: ANOVA, F = 43.60, P = 0.001, 16S: F = 14.5, P