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The alternatives remained on the screen until the participant chose one of the rectangles, which was then highlighted by a thick red outline for 500 ms. After a subsequent interval of 800�C1200 ms, the participants received feedback, lasting 1000 ms, which indicated whether he/she gained (when the valence of the outcome was ��+��) or lost (when the valence of the outcome was ��-��) in that particular trial (see Figure ?Figure11). The formal task consisted of six blocks of 64 trials each. Unbeknownst to the participants, the outcomes were provided according to a predetermined pseudorandom sequence, and each participant received exactly 64 of each kind of outcome for each beneficiary. Each participant was paid 15 CNY for their participation in the study. In Thymidine kinase the gambling task, each beneficiary had 15 CNY in his/her account. Based on the points gained for each beneficiary, the final gain or loss was added to the separate account (every additional 500 points gained increase payment 5 CNY). The total payment for each participant was approximately 60.6 CNY (range, 4075 CNY; SD = 5.6 CNY). FIGURE 1 The sequence of events within a single trial in the monetary gambling task. In each trial, the beneficiary information lasted for 3000 ms then the fixation point lasted for 1200 ms. The participant was then presented with a choice Nutlin-3 research buy of two alternatives, ... Before the experiment, each participant was instructed about the rules and meaning of the symbols in the task. The participants were instructed that the money would be put on the friend��s cell phone or served as class fee. The participants were also encouraged to respond in such a way to maximize the total amount for each beneficiary. The participants were told that the higher the amount earned for each beneficiary, the more bonus money the beneficiary would receive at the end of the study. After the participant finished the task, he/she was told that the task had no optimal strategy. Electrophysiological Recording and Measures Electroencephalographic (EEG) activity was recorded from 63 scalp sites using tin electrodes mounted in an elastic cap (Brain Products, Gilching, Germany) with an online reference to the middle at FCz at the standard locations according to the international 10�C20 system and off-line buy ABT-737 re-referenced to the average reference. The horizontal electrooculogram (HEOG) was recorded from an electrode placed at the outer canthi of the right eye. The vertical electrooculogram (VEOG) was recorded from an electrode placed above the left eye. All inter-electrode impedance was maintained at