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All patients or their legal representatives (if a patient has died) gave written informed consent to participate in the study and to use data from the medical records. For each patient, the following variables were recorded: demographics (age and sex), aetiology of chronic renal failure, RT-related data, management of post-transplant anaemia excluding the early postoperative period, associated co-morbidities developed during the 12 months before dialysis treatment and outcome (hospital admission check details and death) during the first 90 days after restarting dialysis. Moreover, clinical, laboratory data, immunosuppressive regimen and management of anaemia were recorded at 3-month intervals during the 12-month period before readmission to dialysis click here treatment (0, ?1, ?3, ?6, ?9 and ?12). Anaemia was defined as a haemoglobin concentration (MDRD)-4 formula. A decline in renal graft function was defined as an unexplained rise of 25% in serum creatinine clearance compared with baseline. Student's t-test or the Wilcoxon signed-rank test was used for the comparison of haemoglobin levels at the onset of dialysis and for each study period before readmission to dialysis therapy. Differences in morbidity and mortality according to the presence of anaemia and renal function data (serum creatinine, GFR and creatinine clearance) were also analysed. We used multivariable logistic regression to assess the association of clinical factors Amiloride and outcomes in both cohorts. Also, all the patients were followed up until 3 months after graft loss date. Death at third month was analysed as the dependent variable and age >55 years at the time of inclusion, previous diabetes diagnosis, hepatitis C virus (HCV) infection, hypertension, serum haemoglobin level at Month 0, renal function as serum creatinine at Month 0 and use of ESAs were entered in the model as independent variables. The SPSS program, versions 8.0 and 13.0 were used to analyse data of the 2001 and 2009 cohorts, respectively. Statistical significance was set at P