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It may be crude, but it's robust, and if used in obvious cases, can become the ��barn door�� test: all that's needed. Later editorials will deal with using samples for statistical comparison, and other frequently used statistical tests. The authors acknowledge helpful comments from Douglas Curran-Everett during the preparation of this editorial. ""The southern catfish exhibits the largest decrease in critical swimming speeds (Ucrit) during digestion among the fish species that have been investigated. To test whether the maximum metabolic capacity of the southern catfish was improved after exercise training to alleviate the competitive interaction between digestion and swimming, we measured postprandial ALG1 metabolic responses, Ucrit and oxygen consumption rates (MO2) during swimming in both fasting and digesting fish. Twenty-one days of training (50?min swimming at 60% Ucrit followed by 10?min chasing) did not produce significant differences in resting MO2 (MO2rest) or postprandial peak MO2 (MO2peak). However, it did result in a significant decrease in energy expenditure during digestion. Feeding caused a significant decrease in Ucrit and an increase in active MO2 (MO2active), whereas training caused a significant increase in Ucrit but no significant change in MO2active. Neither digestion nor training had a significant effect on metabolic scope (MO2active?MO2rest). Training had no interactive effect on postprandial changes in any measured variable, so we conclude that training did not alleviate the competitive interaction between digestion and swimming. Our results suggest that: (1) the metabolic capacity of GABA activation nontrained fish cannot support the metabolic demands of both digestion and locomotion simultaneously, and swimming metabolism, therefore, is sacrificed to sustain digestion when feeding and locomotion are combined (digestion-prioritization Cobimetinib mode); (2) the metabolic capacity and metabolic mode of competition did not change after training, but trained fish did exhibit improved swimming performance, possibly due to their increased rate of O2 extraction. J. Exp. Zool. 313A:557�C563, 2010. ? 2010 Wiley-Liss, Inc. ""1. Hyperhomocysteinaemia (HHcy) is associated with endothelial dysfunction and has been recognized as a risk factor of cardiovascular disease. The present study aimed to investigate the effect of homocysteine (Hcy) on endothelial function in vivo and in vitro, and the underlying signalling pathways. 2. The HHcy animal model was established by intragastric administration with l-methionine in rats. Plasma Hcy and nitric oxide (NO) concentration were measured by fluorescence immunoassay or nitrate reductase method, respectively. Vasorelaxation in response to acetylcholine and sodium nitroprusside were carried out on aortic rings. Human umbilical vein endothelial cells (HUVEC) were treated with indicated concentrations of Hcy in the in vitro experiments.
