What's Actually Going Down With MK-2206
MicroCT Analysis After in?vivo implantation, pellets from two MSC donors (n?= 2 donors, four pellets per donor and condition) were analyzed by the Quantum FX ��CT scanner (PerkinElmer) using the following settings: isotropic voxel size of 10?��m, 70 kV, check details 160 mA, 5?mm field of view, 3?min scan (FINE setting) per sample. Raw ��CT images were converted into 3D reconstructions using Quantum FX ��CT software (PerkinElmer). Statistical Analysis Data were analyzed with PASW Statistics 20 (SPSS). The normal distribution of data was confirmed using the Kolmogorov-Smirnov test. An unpaired t test or a generalized mixed model was applied; the different conditions were considered a fixed parameter and the donors as a random factor. In the case of multiple comparisons, Bonferroni post hoc test was performed. The extra sum of square F test was used to compare the polynomial regressions shown in Figures 2C and ?and3B.3B. A Levene��s test verified the equality of variances in the samples (homogeneity of variances). p?Ibrutinib clinical trial and design; R.N., M.A.C., N.T., and M.J.H. were responsible for the acquisition of data; R.N., D.t.B., M.A.C., P.A.J.B., and G.J.V.M.v.O. were involved in interpretation of data and preparation of the manuscript. All authors approved the final version of the manuscript. Acknowledgments J. van der Stok, N. Kops, J. Lehmann, and J.L.M. Koevoet are acknowledged for technical assistance. This work was funded by the Netherlands Institute of Regenerative Medicine (grant FES0908) and Science Foundation Ireland (grant 11/RFP/BMT/3150). R.N. was further supported TRIB1 by Translational Adult Stem Cell Research by ZonMw (grant 116005009), and D.t.B. was further supported by TI Pharma (grant D5-402) and Marie Curie (grant FP7-PEOPLE-2009-RG-256560). R.N., M.A.C., and G.J.V.M.v.O. performed the research within the framework of the Erasmus Postgraduate School Molecular Medicine. Footnotes This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Supplemental Information Document S1. Supplemental Experimental Procedures, Figures S1�CS5, and Tables S1 and S2: Click here to view.(593K, pdf) Document S2. Article plus Supplemental Information: Click here to view.(4.1M, pdf)""One key etiological factor underlying a wide range of diseases is the progressive decline in immune function with age (Dorshkind et?al., 2009). At its core is a reduction in lymphopoiesis within the bone marrow (BM) and thymus (Miller and Allman, 2003; Rodewald, 1998), attributed in part to a decrease in the number and function of lymphoid progenitors (Min et?al., 2004, 2006).
