We applied both human endothelial and murine endothelial cells and observed a substantially larger WFA-induced growth inhibition in endothelial cells cultured in STS-CM than in control medium
vitro transcription reaction to produce the biotinylated, amplified target cRNA used for the Illumina RatRef- Materials and Methods Kainate treatment of the rats P Microarray data analysis Microarray data analysis was applied to identify those genes that had statistically significant differences in their expression between the groups. The outliers were excluded based on their expression intensity within a group, and those samples did not affect the outcome of the further analysis. In order to distinguish the two groups under assessment, phenodata was created, and the normalization of genes was made by the quantile normalization method, with no production of flags on the RatRef- Tissue collection Seizures in Juvenile Rats gene ontology classification were obtained using the DAVID Bioinformatics Resources from the National Institute of Allergy and Infectious Diseases, NIH. Immunohistochemistry Fluoro-Jade B staining FJB, an anionic fluorescein with excitation peaks at Hematoxylin-Eosin staining The hematoxylin-eosin staining was performed using basic histological staining protocols. Briefly, brain slices were stained with Mayer's Hematoxylin, dehydrated in ethanol series, and cleared in xylene before using the LCM. May Seizures in Juvenile Rats Results Selective neuronal damage after SE in the CAThe extent of regional hippocampal neuronal damage after KAinduced SE was assessed using FJB staining. Fig. Microarray data analysis profile Transcriptional responses of the CA the cytochrome c oxidase complex. The most significantly influenced pathway was that for the oxidative phosphorylation implicating that the primary responses to the excitoxic damage after KA-induced SE involves oxidative mechanisms. The expression of calmodulin Gene changes associated with gliosis and inflammation. The structural astrocytic protein GFAP was the most dramatically up-regulated gene after SE. The expression of the protease inhibitor cystatin C gene was pronouncedly up-regulated together with one of its substrates, cathepsin S. Also the annexin AGene changes associated with synaptic transmission. The c-aminobutyric acid type A receptor a Genes related to pathways The subsequent KEGG-test for the probe set over-representation analysis revealed the Gene changes related to neuronal damage and calciumbinding proteins. Only a few genes involved in the apoptotic subunit gene expression was down-regulated, whereas the expression of cValidation of selected genes of interest by immunohistochemistry. The validation of the microarray results was carried out by immunohistochemical detection of the selected genes of interest, i.e. GFAP, apo E, CBMay Seizures in Juvenile Rats magnification, highly enhanced PEP-May Seizures in Juvenile Rats Entry number rno Term or pathway Oxidative phosphorylation Proteasome Phosphatidylinositol signaling system Ribosome Long-term potentiation Biosynthesis of steroids Inositol phosphate metabolism Alzheimer's disease Protein export VEGF signaling pathway Gap junction GnRH signaling pathway Long-term depression Glycine, serine and threonine metabolism Screening programs should shell out particular attention to more youthful homeless grownups with mental sickness Nitrogen metabolism P Value, Odds Ratio Count Up-regulated Down-regulated doi: May Seizures in Juvenile Rats Discussion In this study we elucidated the consequences of KA-induced SE on gene expression in the CAMay Seizures in Juvenile Rats Entry number Entry number MF Term Storage protein Myelin protein Carbohydrate phosphatase Interferon receptor Number of altered genes doi: by SE at this age.
