Ways diglyceride Could Have An Impact On The Majority Of Us
3 mL of sodium nitroprusside (5 mM) was added to 1 mL each of various concentrations of the samples and then incubated at 25��C for 150 min. Thereafter, 0.5 mL of Griess reagent was added. The absorbance was measured at 546 nm (12). Determination of total phenol content. Appropriate dilutions of the samples were oxidized with 2.5 mL 10% folin-ciocalteau��s reagent AZD0530 (v/v) and neutralized by 2.0 mL of 7.5% sodium carbonate. The reaction mixture was incubated for 40 minutes at 45��C and the absorbance was measured at 765 nm (13). Determination of total flavonoid content. 0.5 ml of appropriately diluted samples were mixed with 0.5 ��L of 10% AlCl3, 50 ��L of 1 mol.L-1 potassium acetate and 1.4 mL water, and was incubated at room temperature for 30 min. thereafter. Absorbance was measured at 415 mm (14). Characterization of carotenoid constituent. Carotenoids extraction was carried out by the modified method of Takagi (15). The pulverized samples were homogenized in 75 mL acetone and kept at room temperature for 1hour in the dark. The homogenate was filtered through filter paper by suction. The extracts were combined and evaporated under reduced pressure and the residue was re-extracted by a mixture of diethyl ether and petroleum ether in equal ratio. The extract was concentrated with a rotary evaporator and dried using anhydrous sodium sulphate. The extracts (1 ��L: 20:1 split) were analyzed for composition by comparison with standards (Aldrich Chemical Co., Milwaukee, W1) on a Hewlett-Packard 5890 gas chromatograph (Hewlett-Packard Corp., Palo Alto, CA) equipped with a derivatized, nonpacked injection liner, a AC-5 capillary column (30m length, diglyceride 0.25 mm column id., 0.25 ��m film thickness), and detected with a flame ionization detector (FID). Data analysis The results of triplicate experiments were pooled www.selleckchem.com/products/GDC-0941.html and expressed as mean �� standard deviation (STD). One way analysis of variance was used to analyze the results and the least significance difference (LSD) was carried out (16). RESULTS The extracts inhibited AChE activity in a dose-dependent manner as shown in Figure ?Figure1.1. However, the IC50 values revealed no significant (P>0.05) difference between the two samples [ESC (IC50 = 5.83 mg/ml), CER (IC50 = 5.70 mg/ml)]. The butyrylcholinesterase inhibitory ability of the samples as presented in Figure ?Figure22 and the IC50 in Table ?Table11 revealed that CER (IC50 = 3.75 mg/ml) had significantly higher inhibitory activity than ESC (IC50 = 7.42 mg/ml). The incubation of rat��s brain homogenates in presence of Fe2+ caused a significant increase (P
