To maintain symmetry, this step was assigned to the dissociation/association of ADP, which is the last hydrolysis product to leave the NBD

This notation is necessary to distinguish ATP ATP amongst the two-nucleotide species, EATP =FATP , 107091-89-4 supplier according to their NBD hydrolytic action, i.e. Effectively, the pathway for the trapping reaction was substituted with i,j for: none, ATP, ADP, ADPPi and ADPVi, according to Figure 2. In this model, the intermediates E ATP and FATP exhibit the same properties regardless of their origin, whether from the priming reaction or a later hydrolytic event. In this particular implementation of the Alternating Cycle, transformation between the two forms was achieved by exchange of ADP from/to the twonucleotide intermediates to/from the one-nucleotide intermediADP ATP ates: EATP continue reading this species (in 10 s) and the large continual-state portion trapped that was documented in the literature [23].