To Those People Who Want To Grasp SRT1720 But Is Unable To Get Rolling

Sections of roots were taken using a hand-held razor blade and examined under a light microscope that was equipped with a digital camera (Nikon Coolpix 4500, Japan). An eyepiece reticle was used to record root diglyceride diameters. The areas of aerenchyma and stele (expressed as a percentage of total cross-sectional area) were determined in each digital picture using ImageJ software (v. 1.38, National Institutes of Health, USA). Cross-sections of first-order lateral roots were also taken when possible. Areas of aerenchyma in leaf sheaths and internodes of stolons Stolon pieces (3�C5 cm length) kept in 50 % ethanol solution were used for determination of aerenchyma in leaf sheaths and internodes of stolons. The greenest stolon per plant was visually selected, and AZD0530 price two to five cross-sections of leaf sheaths and internodes were taken at 1 cm from the first observable node from the root�Cshoot junction. The areas of aerenchyma in each cross-section were analysed as described above. Once morpho-anatomical traits had been recorded, the remaining parts kept in 50 % ethanol were used for determination of dry weight. As cross-sections used for microscopy studies did not exceed 3 % of fresh weight, the remaining non-cross-sectioned parts were also taken into account for dry weight determination. The remaining shoots and roots were oven dried at 60 ��C for 72 h for dry mass determination. Statistical analysis Data were analysed to generate mean values, standard deviation and analysis of variance (ANOVA) using R software (v. 2.15.2) (R Development Core Team, 2012). Log transformation was carried out to ensure normality of data. Differences between accessions were analysed and reported with the least significant difference (LSD) at �� = 0.05. Results Effect of waterlogging on leaf number, leaf greenness and dry mass production Prior to the beginning of the experiment, all accessions showed a similar number of leaves per plant (around 14 leaves). Leaf production increased in all accessions when grown under drained or waterlogged soil conditions (P learn more (Table ?(Table1).1). SPAD values decreased in all accessions of B. humidicola from the beginning of the experiment under drained or waterlogged soil conditions (P