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They do not have any history of diseases. Exclusion criteria were following: 1) patients diagnosed for diabetic mellitus and stroke; 2) any diagnosis of vascular disease, cancer (clinically or by anamnesis), renal disease, liver disease, thyroid disease, and acute or chronic inflammatory disease. Totally ninety-one subjects (n = 91) were recruited and agreed to the participation consent form. The written informed consent proved before participation was approved by the Institutional Review Board of Dong-a University. Anthropometric parameters, blood pressure and blood collection Body mass index (BMI, kg/m2) was calculated Selleckchem Lenvatinib using body height and weight. Blood pressure was measured in the arm of seated patients with an automatic blood pressure monitor (HEM-7220, OMRON, Matsusaka, Japan) after a rest. After overnight fasting, venous blood samples were collected in ethylenediaminetetraacetic acid (EDTA) - treated and plain tubes, separated into plasma and serum, and then stored at -80�� until analysis. Serum lipid profile Fasting total cholesterol and triglyceride levels were measured using commercially available kits on a Hitachi 7150 Autoanalyzer (Hitachi Ltd., Tokyo, Japan). After precipitation of serum chylomicrons with dextran sulfate magnesium, the concentrations of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) cholesterol in the supernatants were enzymatically measured. Fasting glucose, glycated hemoglobin (HgbA1C) and liver function Fasting glucose levels were measured using a glucose oxidase method with a Beckman Glucose Analyzer (Beckman PIK-3 Instruments, Irvine, CA, USA). Glycated hemoglobin (HgbA1C) was measured using Variant II Turbo HgbA1C kit-2.0 (Bio-Rad, Hercules, CA, USA) on Variant analyzer (Variant II TURBO, Bio-Rad, Hercules, phosphatase inhibitor library CA, USA). Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) were measured by a Modified IFCC UV method. Data analysis Statistical analyses were performed using SPSS version 21.0 for Windows (SPSS Inc., Chicago, IL, USA). Spearman and partial correlation analyses were performed for the relationships of fasting glucose or HgbA1C with basic and biochemical parameters. Skewed variables were log transformed for statistical analysis. For descriptive purposes, mean values were presented using untransformed values. Results were expressed as means �� SE or percentages. A two-tailed p value