These data demonstrate that PMA prolonged recovery from "slow" inactivation subsequent physiologically relevant stimulus trains as properly as next sustained move depolarization

Reliable strains demonstrate exponential fits to the imply information (-120mV Cells were collected and washed three times with cold PBS (free of Ca2+ and Mg2+) and then stored at -30 keeping A = .seventy nine, t = 24.eight s -100mV holding A = .eight, t = fifty four.seven s -eighty mV keeping A = .sixty two, t = 103.2 s). 69 3.6% in PMA n = 4 p = 0.21, paired t test) (Fig 4A). The recovery was biphasic, and Fig 3. PMA significantly prolonged recovery from inactivation in 2a containing channels. (A) HEK293 cells were transfected with CaV2.2, 2, and 2a subunits. Inactivation was produced by a 10s prepulse and recovery tracked as in Fig 1F. This was done twice in each cell: in some cells PMA was applied 5-min before the second stimulation protocol (left panel), while other cells were used as time-matched controls (i.e. no PMA was applied right panel). Solid lines show exponential fits to the mean data (left panel: control A = 0.82, t = 40.4 s PMA A = 0.65, t = 101 s, comparison of fits F = 24.2 p