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Intraocular pressures were measured by Tonopen XL pneumatonometer (Reichert Ophthalmic Instruments) at 1, 2, and 3 months. Enucleation and Examination The animals were Panobinostat supplier euthanized 3 months after implantation using U.S. Food and Drug Administration (FDA)�C and Institutional Animal Care and Use Committee�Capproved procedures. The eyes were then enucleated, and care was taken not to disturb the bleb and the implant. The bleb was then sectioned through the middle of its roof and the AGV removed. The thickness of the bleb roof in each section was measured with digital calipers (resolution 10 ��m, accuracy ��30 ��m; VWR International, Radnor, PA) as shown in Figure 3. The AGV was then visually examined for the presence of attached polymer. Ten percent formalin was injected into the vitreous cavity, and the enucleated eye was stored overnight in 10% formalin. Histologic sections of the bleb and surrounding tissue were prepared and examined using light microscopy by a pathologist (CEM) who was blinded to the different groups. Figure 3 (A) Enucleation of eye and bleb after 3 months and (B) measurement of bleb roof with calipers. Statistical Analysis Statistical analysis was performed by a statistician (DZ) who was blinded RHOBTB1 to the different groups. One-way analysis of variance (ANOVA) followed by a posttest based on Student-Newman-Keuls analysis was used to compare the experimental groups to the group with a GDD modified with PLGA alone. Results There were no instances of infections, unexpected animal deaths, or other complications. Of the 48 eyes, 47 operations resulted in the formation of an elevated, vascular bleb; one operation, in the PLGA 1.35-mg 5-FU group, did not, due to improper placement of the AGV tube at click here the time of surgery. This eye, which failed due to surgical error unrelated to the experimental model, was excluded from this published analysis; however, including it in an auxiliary analysis did not change any measures of statistical significance. Intraocular pressure showed significant group differences (F = 2.98, P = 0.02) preoperatively in control versus P(HEMA) (mean difference ?4.5 mm Hg; P = 0.013) and in control versus PLGA 0.45-mg 5-FU plus 0.65-��g MMC group (mean difference ?5.75 mm Hg; P = 0.002). At 1 month, significant group differences (F = 5.01, P