The formed crystals in the cells were volatilized by incubating with 150 dimethyl sulfoxide for 10 min at room temperature and quantified by measuring the optical density at 490 nm using an enzyme-linked immunosorbent assay reader

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Steady with these buy SB 202190 benefits, transfection of miR-203 mimics diminished the endogenous expression of ZNF217 at the two mRNA and protein amounts in SW480 cells (Fig. 4B), as well as invasion of SW480 cells. Inversely, downregulation of miR-203 in Figure 1. IHC staining of ZNF217 in CRC. (A-Left) Cytoplasma staining of ZNF217 in CRC cells. (A-Right) Lack of ZNF217 expression in normal colonic epithelial cells. (B) The percentage of positively stained cells in cancer and adjacent normal tissues (P PP 242 reports.