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Student��s t-test revealed significant difference between the ASSO�CFFQ and the WFR for all nutrients, except for vit B12 and folate. Exponentiated mean differences were found to be higher than 2 only for SFA, fructose, sucrose, starch, niacin, ethanol, and caffeine (data not shown). For energy intake, a mean ratio of 1.54 was found this website out, showing that energy intake estimate provided by the ASSO�CFFQ was on average 54% higher than the real value. Bland�CAltman plots for energy and all nutrients showed that differences in intake are distributed quite well around their mean, and they were within the LOA for almost all subjects (only a few units are placed outside), even though the LOA of almost all nutrients were wider than the threshold suggested above. In general, 35 out of 52 nutrients showed at least 90% of units within the LOA and this is reported in Fig. 2 for energy, carbohydrate, calcium, and vit C. Fig. 2 Bland�CAltman for the comparative validity analysis of energy, carbohydrate, calcium, and vitamin C. The solid horizontal lines indicate the mean difference between the two methods and the broken horizontal lines indicate the lower and upper Quinapyramine Limits ... Spearman��s cc and crude and E-adj Pearson��s cc are shown in Table 4. The threshold of 0.4 was overtaken only by lactose, while for all the others nutrients unadjusted Spearman��s and Pearson��s (on transformed data) cc ranged from 0 (several nutrients) to 0.38 (DHA and ethanol); however, most of those cc were not significant. Applying the adjustment for energy intake, cc values became significantly fair (cc��0.25 and Neratinib cell line and arachidonic acid and vit A cc increased for males (Table 4). Raw Pearson��s cc became fair/high (��0.25) for energy and 28 nutrients (almost all fats, carbohydrates, vitamins, and minerals); after adjustment for energy intake, 13 Pearson��s cc for females were found to be higher. Discussion In the present study, the comparative validity of a new web-based FFQ in measuring food consumption and energy and nutrients�� intake was evaluated. Results showed that the tested FFQ is an appropriate tool for ranking adolescents in classes of food groups, even though it is not suitable for measuring the absolute intakes of all food groups. These results are in line with other studies (4�C8, 21�C24). Different analytical procedures were used to estimate the validity of the ASSO�CFFQ.