The Straightforward Truth Regarding PTEN
The plaque may be seen in situ only after application of disclosing solution or by using the probe on tooth surface) were included into the study by obtaining written informed consent. Subjects who did not meet criteria and who are under antibiotic therapy were excluded. Using random allocation rule participants were divided into chlorhexidine group, green tea group, and plain water group. Cards with group names were kept in sealed envelopes, and all the participants were asked to pick up the sealed envelopes. Envelopes are opened by investigator only after the participant��s name was written on the appropriate envelop. Before starting intervention a base line Belnacasan plaque samples were collected, 10 ml of each solution were given to the respective groups to rinse for one minute. Plaque samples were collected at five minutes after rinsing. A wash out period of seven days were given and all the subjects were recalled for repetition of above procedure. Hence all the 30 subjects were exposed to all the three rinses with a wash out period of seven days between interventions [Table/Fig-1]. Plaque samples were collected using sterile buds from lingual side of lower molars and this sample were transferred to sterile test tube containing thioglycolate transport media. All the samples were sent for microbiological analysis within half an hour of sample collection. [Table/Fig-1]: Flowchart of methodology Microbiological PTEN Analysis Microbial analysis was done in Department of Microbiology, Government Medical College in South GF109203X ic50 India. Plaque samples were diluted with sterile saline in a ratio of 1:1000 and streaked on chocolate agar plates, this plates are incubated for 48 hours at 37��C and number of bacterial colonies counted [Table/Fig-2a,b]. [Table/Fig-2]: Counts of S.mutans were expressed as no. of colony forming units/ml, * Statistically significant Statistical Analysis Mann-Whitney U test and Wilcoxon matched pairs test were used and data was analysed using SPSS software (version 16). A ��p�� value of
