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Participants were asked Ficain to fixate at 5 points marked on the cross using a cue. Calibration was performed separately for each video. Figure ?Figure33 shows an image of the setup of the calibration. The calibration procedure was done using a graphical user interface (GUI) developed in MATLAB (Javadi et al., Unpublished). We should emphasize that this is a simplified method of detection of the PoR. For more elaborate methods, one should consider many other parameters such as glint and eye model (for a review see Morimoto and Mimica, 2005). Figure 3 Calibration cross (on the left) used for calibrating the eye and scene cameras to map pupil center point (PCP) to point of regard (PoR). Statistical analysis Images in each collection (��Natural�� and ��CASIA-Iris��) were manually processed by two experimenters (AHJ and LT) independently, in order to detect the PCP in each frame using a graphical user interface (GUI) developed in MATLAB. The mid-point in-between their selected PCP for each image was used for further analysis, pm (xm, ym). pm (xm, ym) were considered as the ground truth and the performance of the algorithms were compared Small molecule library mouse against these points. Detection error for each algorithm (��e��) was calculated by Euclidean distance between detected PCP, pd (xd, yd), and manually selected PCP: e=|(xd?xm)2+(yd?ym)2| (3) Due to the asymmetric distribution of e (e�� 0) and its concentration close to zero, we used a criterion based on exponential decay to label the frames as hit or miss. Exponential decay is described as follows f(x;p0,ps,pr)={p0+psexp(?prx)x��00x0, po and ps are rate, offset and scale parameters, respectively. For further discussion on this method Idelalisib research buy refer to Appendix 2 in the Supplementary Material. We fitted an exponential decay to the data for each model and image collection. e values smaller than x-intercept, (po + ps)/pr ps, of the line describing the slope of the decay were marked as hit. The rest were marked as miss. Only images marked as hit were used for further analysis. Processing time was also recorded for each method in their native programming environment, i.e. MATLAB for Starburst and C# for Gaze-Tracker. Although the detection errors are consistent across platforms, the processing speed is not. Therefore, to have a valid comparison between methods, we compared the speed of SET in MATLAB with Starburst and in C# with Graze-Tracker. Processing times (in milliseconds) further than 2 standard deviations from the mean for each method and image collection were excluded from the analysis. Statistical data analysis was performed using SPSS (v17.0; LEAD Technologies Inc., Charlotte, NC, USA). Data was checked for normality of distribution using Kolmogorov-Smirnov test. The test of normality showed that the distribution of detection errors was not normal in any of the methods and image collections (in all the comparisons: p

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