The Historical Past Behind The UBE3A Triumph

We also developed a method for efficient transgenesis. These studies demonstrate that P.?waltl newts are a suitable model animal for analysis of regeneration using molecular genetics. Establishment of this experimental model will enable us to perform comparable studies using these newts and other vertebrate models. Regeneration, which enables tissue reconstruction after injury, has been studied for many years. Although a lot of research has been performed, the mechanisms of regeneration at the genetic and molecular levels are mostly unclear. In order to study gene function in vivo, molecular genetic analyses find more are very powerful. It involves gene manipulation, such as transgenesis and knockout, in addition to the analyses of mutants. In particular, it is important to establish transgenic or knockout (mutant) lines, and systematically cross these lines to study the functions of the genes. In fact, such systems have been developed for vertebrate models of regenerative research such as Xenopus, Axolotl (Ambystoma mexicanum; neotenic salamander; belonging to family Ambystoma), and Zebrafish. However, there is currently no experimental model system using Trichostatin A order molecular genetics for newts (family Salamandridae, subfamily Pleurodelinae; not including Axolotl). Newts have the remarkable ability to regenerate lost tissues, such as limbs, optical tissues (lens, retina and cornea), brain, spinal cord, intestine, and heart (reviewed in: Brockes & Kumar 2002; Agata & Inoue 2012). Of the vertebrate models mentioned above, only newts are able to regenerate all of these organs, and the regeneration is observed throughout their life even after metamorphosis. Thus, establishment of molecular genetic systems using newts would represent valuable UBE3A experimental models for studies involving regeneration, stem cells, and reprogramming. However, newts, which are commonly used for regenerative research, are not currently suitable as the model animal in molecular genetics due to difficulties encountered with their large-scale breeding. For example, the maturation of sperm and eggs is seasonal, and each female only spawns