The Double Twirl On Staurosporine

Ultrastructural analysis of TIPS microcarriers Scanning electron microscopy (SEM) was used to assess the surface topography of PLGA microcarriers. Cellularized microcarriers were fixed in 2.5% glutaraldehyde (Sigma-Aldrich) in PBS for 15?min, followed by dehydration through Selleck Staurosporine increasing concentrations of ethanol (70%, 80%, 90%, 100%; Sigma-Aldrich). The samples were transferred to hexamethyldisilazane (Sigma-Aldrich), mounted on aluminum stubs using adhesive carbon tabs, and sputter coated with gold/palladium (Polaron E5000). Samples were viewed under a JEOL JSM-5410 LV SEM (JEOL) at 20?keV. Statistical analysis All experiments were repeated in replicates of five, unless otherwise stated. The data were analyzed using the unpaired two-tail Student's t-test and one-way analysis of variance with Dunnett's multiple Resminostat comparison test using GraphPad Prism v.4.00 statistical software (GraphPad Software). The data are presented as mean��standard deviation. A p-value of selleck kinase inhibitor PCR was performed using relative quantification. All data were normalized to the housekeeping gene and compared to the threshold cycle (CT) value of the calibrator obtained at the beginning of the differentiation process (day 12 postseeding). The expression of caldesmon, calponin, and myosin heavy chain was increased significantly after 7 and 14 days of culture in the differentiation medium (Fig. 2 and Table 1). Cells cultured on TIPS microcarriers incubated in the control medium did not express increased levels of these markers at either time point. FIG. 2.