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Expression of Th2 and Th17 mRNA markers increased in the middle part of the small intestine of whey-sensitized mice, which was prevented by GF. By contrast, GFA enhanced Tbet (Th1), IL-10 and TGF-�� mRNA expression compared to GF which was maintained in the distal small intestine and/or colon. Dietary supplementation with scGOS/lcFOS or scGOS/lcFOS/pAOS during sensitization, both effectively reduce Selleckchem Doxorubicin allergic symptoms but differentially affect mucosal immune activation in whey-sensitized mice. ""Sputum eosinophil counts and eosinophil cationic protein (ECP) levels are usually increased in asthmatic patients. The correlation between sputum eosinophils or ECP and clinical findings of asthma has been previously investigated but many of these studies have been performed on small samples of asthmatic patients, considering only few clinical indices and often including patients on oral or inhaled corticosteroids, which might be confounding when interpreting the relationship between disease activity and airway inflammation. To assess whether sputum Obeticholic Acid molecular weight eosinophils and ECP were differently related to functional and clinical parameters of asthma in a large number of steroid-na?ve asthmatic patients, taking into account several potential determinants of activity and chronicity of asthma. One hundred and twenty-nine patients with mild�Cmoderate asthma were studied. Sputum was induced by hypertonic saline inhalation and processed using the whole sample method. Sputum eosinophils and FARP1 ECP significantly correlated with each other (r?=?0.41, P?