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3). This analysis revealed no significant differences between the results from each method. Therefore, the methodology of MSPE is comparable with the reference methodology. Additionally, the MSPE is a robust preconcentration technique that can be coupled even to spectrophotometry or HPLC. Figure 6 Chromatograms of (a) original sample and (b) eluted tartrazine solution after using MSPE developed method. Table 7 Contents of tartrazine (mean and %RSD, n = 3) in real samples determined with the proposed methodology and comparison with HPLC reference method. 4. Conclusions In the present work, an activated carbon E-64 covered with magnetite support was synthesized; this support has magnetic properties that allow its separation by applying an external magnetic field. The best conditions for the extraction and elution of tartrazine were an initial sample volume of 20?mL, buffered with acetate buffer solution, at pH 5, and mixed with 75?mg of magnetic modified carbon, tartrazine elution with 1?mL of NaOH 0.25?mol?L?1 in methanol. Thus, the proposed sample treatment coupled to spectrophotometric analysis is an alternative to the analysis of azo dyes in the food industry because the parameters, analytical precision, and accuracy are similar to HPLC methodologies. However, JQ1 order the proposed methodology saves time and is less expensive than the reference method. Acknowledgments The authors wish to thank CONACyT (Project INFR-2014-227999) and Consejer��a de Cultura, Educaci��n e Ordenacion Universitaria, and Xunta de Galicia (Project EM 2012/153) for the financial support. Alfredo Guevara-Lara, Ma. Elena P��ez-Hern��ndez, Selleck R428 and Jos�� A. Rodr��guez gratefully thank the SNI for the distinction of their membership. Conflict of Interests The authors declare that there is no conflict of interests regarding the publication of this paper.""It is increasingly important to profile proteins in order to understand biological processes in a postgenomic era as the dynamics of proteins between cells at different times and under different environmental conditions provide an actual biological phenotype. In particular, the presence of posttranslational modifications in proteins further highlights the importance of proteomic analysis which is not replaceable by other genomic approaches [1]. To profile the proteome of tissue samples, the proteins have to be extracted using relevant solvent. Currently, there are two major approaches to prepare the tissue samples for proteome analysis. The first approach, termed as gel-based separation and in-gel digestion, involves the use of detergents like SDS to solubilize the proteins before separation by SDS-PAGE and subsequent digestion of the proteins trapped in the gel [2]. The second approach, termed as in-solution digestion, involves the use of strong chaotropic reagents like urea and thiourea to solubilize the proteins before digesting the proteins in the solution [3].
