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cse.ucsc.edu/goldenPath/currentGenomes/). The assembly versions were yeast (SacCer_Apr2011), roundworm (WS220/ce10), mouse (GRCm38/mm10) and human (hg19, GRCh37). For the insect species, the names have been abbreviated as the first letter of the genus followed by three letters of the species name throughout the text and illustrations. 2.2. Identification of imperfect microsatellites The imperfect microsatellites Epigenetics Compound Library chemical structure were identified from the genome assemblies using the SciRoKo 3.4 program, employing the approach that implemented fixed penalty motif mismatch criteria.23 The minimum score 15 and mismatch penalty 5 were used as search criteria to identify imperfect microsatellites of mono-, di-, tri-, tetra-, penta- and hexanucleotide repeats in each species. We also used slightly different parameters such as a minimum score of 15 and mismatch penalty of 3, and a minimum score of 10 and mismatch penalty of 5 to search microsatellites BMS-777607 manufacturer to investigate whether changes in parameter values affected the results of this study. In none of the searches, more than three consecutive mismatches per locus were allowed to identify the imperfect microsatellites. 2.3. Motif mismatches and microsatellite length The number of imperfect microsatellites along with their length and number of mismatches of individual locus, as predicted by SciRoKo, were used for different comparative analyses among the species. The genomic abundance of imperfect microsatellites Evodiamine with different length and motif mismatches was chosen for different comparative analyses. First, we targeted microsatellites of length our search conditions. Similarly, microsatellites above 25 bp in length may have two mismatches as per our search conditions. Second, we targeted longer microsatellites (35, 40, 45, 50, 55, 60, 65, 70, 75, 80 and 85 bp) to compare repeats that have different number (1, 2, 4 or 6) of mismatches. The third comparison was made between microsatellites that were at least 30 bp long and had

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