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3 �� 4.9 years; Non-Professional 1st Division) Sub-Elite (SE; n = 35, age, 24.3 �� 4.2 years; Non-Professional 2nd and 3rd National Divisions) Moderately Trained (MT; n = 32, age, 24.2 �� 5.1 years; Regional Championships). All participants were tested during the 2008 - 2009 Portuguese HB season (February and March 2009). 3.2. Measuring Protocol Participants were measured in two basic measures (stature and body mass), and performed six fitness tests, of which were recorded eleven variables for analysis (30 ms print time, handgrip-dominant, non-dominant, and difference between both; squat jump height-SJ, countermovement jump Resminostat height-CMJ, leg power in jumps-Pavg, number of repetitions in 60 seconds sit-ups, and the distance and the position achieved in the Yo-Yo IE2). Stature (cm) was measured using a portable anthropometer (GPM, Siber-Hegner, Switzerland), and body mass (kg) was measured, to the nearest 0.5 kg, using a scale (Secca model 761 7019009, Vogel and Halke, Germany). Before the fitness tests, all participants performed a 20 minutes warm-up. Participants performed six fitness tests (following the order established in the description) and eleven variables were recorded for analysis. The tests included a 30 minute speed test. Participants completed three Dorsomorphin nmr trials and the best score (time in seconds) was recorded for analysis. All sprint times were recorded using electronic timing lights (Wireless Sprint System, Brower Timing Systems, Salt Lake City, Utah, USA). The players performed two vertical jump tests, on an Ergojump (Bosco System, Globus, Italy) using the Bosco protocol, to determine lower body explosive strength. Three trials of each test (squat jump, SJ; countermovement jump, CMJ) were performed and, the best trial result of each was recorded (height; in cm). Leg power was also assessed (Pavg, in W), using a modified version of the Lewis formula. Abdominal strength (i.e. endurance) was assessed using the 60 s sit-up test. Handgrip was assessed using a grip strength dynamometer (Grip Takei Physical Fitness Test-T.K.K. 5001, Staurosporine chemical structure Japan). Again, the participants completed three trials, with each hand. The best scores (in kgf) were recorded and the difference between dominant and non-dominant handgrip (Handgrip, D-ND) was calculated. The participants completed one trial and the number of repetitions (#) was recorded. Finally, to study intermittent endurance capacity, the Yo-Yo Intermittent Endurance Test-Level 2 (YYIE2) was used. The distance (in meters) and the position achieved (using a four point scale where 1, 2, 3, 4 represents respectively,
