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All animal experiments were performed according to national and international guidelines and laws of animal welfare	All animal experiments were performed at the animal facility of the USTL Lille according to the rules of the European Community Council guidelines	All animal procedures had been carried out in accordance with european legislation for animal experimentation (Directive 86/609/EEC) and with french laws on animal research
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All dilutions ended up operate for every gene and the most dilute cDNA samples that successfully amplified the gene of interest (10-2 or 10-3) had been utilised for quantification	All doses and concentrations of rivastigmine are expressed in mM or mg/kg of the tartrate salt did not have a cytotoxic result on activated cells	All endeavours were created to lessen struggling
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All of the procedures involving the animals conformed to the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research and was approved by the Committee on Ethics in the Care and Use	All of the six emergence factors considered (Table 1) play a role in the changing Campylobacter epidemic, demonstrating the multifactorial nature of this infectious disease	All of these Wee1-like kinases share a comparable construction with a C-terminal kinase domain and a much less conserved, N-terminal domain
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All participants underwent the regular procedures on the study protocol including assessment of healthcare history, smoking status and alcohol consumption habits, physical examination, routine blood tests, and an oral glucose tolerance test	All picture analyses had been performed by observers who were blinded to the therapy circumstances	All rats were maintained in a room equipped with an air-filtering system, and the cages and water were sterilized
All round, our genetic examine favors the speculation that sporadic EOCRC is a sub-group of CIN tumors with neither BRAF mutation nor methylator phenotype	All samples were normalized to internal controls and fold changes were calculated through relative quantification	All samples were then analyzed in duplicate and all reactions run on an Applied Biosystems Sequence Detection System 7500 with relative expression levels determined
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