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Care was taken to minimize the area of sand exposed for the measurement to avoid drying, but steady recordings were obtained suggesting PRDX4 that evaporative losses were minimal (Supporting Information Fig.?S2). For the analysis of agar, a scalpel was used to cut 0.2?g sections from the plate, and each was melted in 1.8?g of distilled water (1 in 10 dilution) by heating in a microwave oven. A minimum of at least three replicates were used for each measurement. All statistical analyses were carried out using GenStat (12th edition; VSN International Ltd, Harpenden, UK). For root physiology experiments, means were plotted and general analysis of variance (anova) was carried out followed by comparison Bleomycin research buy of means using the LSD (5%). LSD and SED values are given in Supporting Information Table?S2. Some data were transformed to a log scale to stabilize variance across treatments (figure legend will indicate). Graphs were made using SigmaPlot (version 11; Systat Software Inc, San Jose, CA, USA). The RSA of seedlings grown on agar and sand under the same growth conditions was compared. The PRL of sand-grown seedlings were significantly longer (P?buy Duvelisib both LRN and TLRL significantly decreased (Fig.?3b). The same response to matric potential was observed for LR density, but only for sand with a particle size