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Intrachromosomal fusion events create palindromic sequences, which make identification of the fusion site problematic and thus despite these events being more numerous, we were only able to characterize six such events. Therefore, our subsequent analysis was focused on interchromosomal fusions, and because these were rare in LIG4?/? cells, we used both direct next generation sequencing (Illumina) of telomere fusion products, as well as Sanger sequencing, to obtain sufficient events for analysis. We observed distinct distributions of the fusion events, with a bias toward fusions containing telomere repeats on either side of the fusion point in the LIG3?/?:mL3 cells compared to LIG4?/? cells, where fusion was biased toward subtelomeric deletion (p?PLX-4720 purchase ��2 test) that was intermediate between the fusion profiles of the LIG3?/?:mL3 and LIG4?/? cells (LIG3?/?:mL3:WT, p?learn more a decrease in the extent of microhomology use at the fusion points in LIG3?/?:mL3 cells (mean 1.6 nts) compared to LIG4?/? cells (mean 2.8 nts, p?= 0.0001, t test; Figure?7F). Microhomology use in the WT HCT116 cells (1.9 nts) was intermediate between LIG3?/?:mL3 and LIG4?/? cells. The reduction in mean microhomology use in the LIG3?/?:mL3 cells was also accompanied by Cefaloridine a reduction in the proportion of fusion events that did not use microhomology at all: in LIG4?/? cells, 4.6% displayed no microhomology compared to 26% in LIG3?/?:mL3 cells (p?