Q-VD-Oph Principals Described

In addition, ER-negative tumors were subdivided on the basis of their AR status [25], and cytokeratin 5/6 (CK5/6) staining was employed as a marker of basal-type tumors. Furthermore, we obtained IHC scores for PIP on the basis of the percentage and intensity of staining for this protein in each tumor (Figure?2, A and B). Next, we assessed the association of PIP expression with biomarkers and molecular subtypes. We observed that AR-positive tumors have a significantly higher PIP expression compared to AR negatives (P BAY-61-3606 purchase tumors, PIP expression was associated with AR-positive status and was unrelated to CK5/6 staining ( Figure?2D). It is notable that PIP expression was not associated with either tumor Selleck QVDOph size or grade in this cohort (P > .1). These findings suggest that PIP is widely expressed in luminal A, luminal B, and ER??/AR?+ (molecular apocrine) subtypes of breast cancer. Moreover, PIP expression is associated with AR and is present in both luminal and basal ER-negative tumors. To investigate the functional role of PIP in breast cancer, we carried out a nonbiased genomic approach to study the transcriptional signature of this gene using a microarray data set of 52 breast cancer cell lines [21]. To identify PIP coregulated genes, we first Thymidine kinase calculated the Pearson CC for each gene expression in the data set with that of PIP and then obtained the list of genes that had Pearson CC values ��?0.5 (P