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Regarding the cerebellum, we first analysed cFos Neratinib purchase expression in the granule cell layer of different vermal lobules. As revealed by a one-way multi-variate analysis of covariance (MANCOVA), the treatment group produced an effect that approached, but did not reach, statistical significance (Wilks?=?0.14 F24,41?=?1.61, P?=?0.08), whereas the number of pairings did not even have a trend towards producing any relevant effect (Wilks?=?0.59, F8,14?=?1.20, P?=?0.36). These results prompted us to analyse cFos expression in further detail, then separating the functionally distinct apical and medial regions of the granule cell layer of different cerebellar lobules (Figs?1, 4 & 5). A one-way MANCOVA revealed a significant effect of the group (Wilks?=?0.11, F24,41?=?1.93, P?BMS-777607 mw but not of the number of pairings, which was used as covariate (Wilks?=?0.56, F8,14?=?1.35, P?=?0.29). Subsequent univariate analyses showed a significant effect of the group in all cerebellar vermis lobules (P? and that this relationship might be more prominent in some specific lobules. On the other hand, a separate one-way MANCOVA RVX-208 comparing cFos expression in the medial region of the granule cell layer also revealed an effect of the group (Wilks?=?0.11, F24,41?=?1.94, P?