Human airway basal cells were infected with lentivirus expressing GFP (Lenti-GFP) or NICD1-4 (Lenti-NICD1-4) and cultured on ALI for 28 days
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Relative to Lenti-GFP contaminated cells, there was a significant lower (p.9) in expression. In contrast, there was substantial increased expression of HES4 (p.five and five.% Lenti-GFP vs five.two% Lenti-NICD4, p>0.five, Fig. 6B) and ciliated cells (17.one% Lenti-GFP vs sixteen.6% Lenti-NICD2, p>0.5 and 17.one% Lenti-GFP vs fourteen.seven% Lenti-NICD4, p>0.08 Fig. 6C). In distinction, constitutive activation of Notch signaling via expression of NICD1 or NICD3 resulted in a considerable improve (both p.two), but a important decrease in expression in cells contaminated with Lenti-NICD2, NICD3 or NICD4 (all p.two) in expression of MUC5AC and SCGB1A1 were noticed for Lenti-NICD4. Astonishingly, a important improve (each p
