How You Can Recognize A Genuine JQ1
In order to optimize the process, possible interactions among other operating parameters should be considered. Response surface methodology (RSM) considers the probable interactions among operation parameters. RSM is an aggregate of statistical and mathematical techniques used for developing, improving, and optimizing the processes [5, 10]. Sonication has been applied for the extraction of polycyclic aromatic hydrocarbons, pharmaceutically active compounds, and flavonoids from different matrices [5, 11, 12]. The aim of this study is to determine the effect of applied R428 cost parameters on poppy straw by using ultrasound extraction method with RSM. 2. Experimental 2.1. Reagents and Materials Papaver somniferum was collected from fields of Opium Alkaloids Factory on the harvesting period in July of 2012 (Turkey). Capsules of the plant were dried at room temperature after the seeds within the plant had been removed. Dried capsules were grinded to the size of 80�C100 mesh before extraction. All chemicals used in experiments were of analytical grade and all chemicals used for analysis were of HPLC grade. Analytical grades NaOH and HCl were purchased from Merck Chemicals. 0.45?��m membranes E-64 (Millipore, Bedford, MA, USA) was used for filtering the buffer solutions. Morphine Sulfate USP Reference Standard JQ1 CII (Cat. number 1448005) was obtained from the Turkish Grain Board (Afyonkarahisar, Turkey), which is the only legal supplier of narcotics in Turkey. Specifications of Morphine Sulfate USP Reference Standard CII (Cat. number 1448005): ? Molecular formula of morphine sulfate is (C17H19NO3)2��H2SO4��5H2O. ? Molecular weight of morphine sulfate is 758.83. ? 132.93?mg of morphine sulfate pentahydrate is equivalent to 100?mg of anhydrous morphine base. 2.2. Ultrasound Assisted Extraction Ultrasound assistant extraction was carried out using Bandelin Sonorex brand ultrasonic bath with 50?kHz frequency. pH levels of the solutions were controlled by Mettler Toledo��Seven Easy pH meter. For the standard ultrasonic conditions, Erlenmeyer flasks were placed inside the ultrasonic bath. Water inside the ultrasonic bath was circulated in order to keep the temperature stable. Solvent level in the Erlenmeyer flask and water level in the ultrasonic bath were kept the same. In order to adjust pH levels to 1 or 13, hydrochloric acid and sodium hydroxide were used. After the extraction process had been completed, mixture was filtered with Whatman filter paper in order to prevent capillary blockage first and was then filtered with 0.45 micron membrane filter (Millipore, Bedford, MA, USA). 2.3.
