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After 5 days, conscious, freely moving mice had their AP recorded for 30 min. Following the recording, the efficacy of the SAD was determined by the lack of bradycardic response to phenylephrine-induced ramp increases in AP. Pulsatile AP recordings were analysed by customized computer software, and beat-by-beat time series of systolic AP were generated. Time series of PI were also obtained by intervals between consecutive values of diastolic pressure. The time series DAPT supplier were obtained for each period of the experiment (i.e. the baseline and after administration of methylatropine or propranolol). For baseline recording, the time domain indices of AP and PI variability were calculated. Overall AP and PI variability were determined by the standard deviation of successive normal values (SDNN) of these parameters. The PI variability was also determined by the square root of the mean of the sum of the square of differences between adjacent PI values (RMSSD; Task Force, 1996). The AP and PI variability were also studied in the frequency domain by spectral analysis. The time series of systolic AP and PI were resampled to 10 Hz by cubic spline interpolation Sulfatase and divided into contiguous segments of 512 values, which overlapped by 50%. After Hanning windowing, the spectrum of each segment of either the systolic AP or PI series was calculated using a fast Fourier transform (FFT) algorithm and integrated in two frequency bands: low (LF; 0.25�C1 Hz) and high (HF; 1�C5 Hz). Data are presented as the means �� SEM. Baseline values of AP and PI were compared among groups using one-way ANOVA followed by Tukey's post hoc test. The same variables were compared before and after the administration of methylatropine or propranolol using Student's paired t test. Doxorubicin chemical structure The SDNN and RMSSD as well as the LF and HF power of PI and systolic AP were compared among groups using ANOVA on ranks followed by Dunn's post hoc test. Statistical significance was accepted when P
