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We successfully extracted miRNAs from FFPE, FS and OCT samples and found that although the RNA in FFPE specimens is considered to be more degraded and damaged than their frozen and OCT-embedded counterparts, the miRNA expression profiles from FFPE samples reliable reflect the information that is retained in those matched from frozen and OCT-embedded tissue samples. Thus, we found a strong correlation between the different preservation methods with correlation coefficients ranging from 0.91�C0.95 (P?Resiquimod weak pattern of clusters with higher correlation coefficients within each preservation method than within matched samples (Fig?2). This might 3-MA indicate that the preservation method to some degree may affect the miRNA expression level and causes us to suggest that only a single preservation method should be considered for a certain experimental set-up. Even though more samples preferable should be run, the fact that the correlations also were observed when using alternative platforms as panel, and individual qRT-PCR confirms the consistency of the results and supports the robustness of miRNA detection in human skin. Recently, we showed that miRNAs obtained from FFPE tissue samples have a high diagnostic potential for skin diseases as we demonstrated that miRNAs can discriminate Cutaneous T-cell lymphomas (CTCL) from benign inflammatory skin diseases (24). Thus, miRNAs offer an appropriate and flexible approach in clinical practices and may hold great promise for biomarker and novel target discovery for skin diseases in the future. We thank S. Boesen, M. M. Petersen and T. Gejsing for excellent technical assistance and N. Bangsgaard for help with psoriatic skin sample collection. This work was supported by The Danish Advanced Technology Foundation (http://www.mirskin.dk) and The Danish Agency for Science, Technology and Innovation. All authors performed the research, analysed the data and wrote the article. J. R. Zibert, N. ?dum, M. A. R?pke and L. Skov designed the research study. M. B. L?vendorf, J. R. Zibert, Compound Library datasheet P. H. Hagedorn and M. A. R?pke are employed by LEO Pharma A/S and C. Glue is employed by Exiqon A/S. Data S1. Materials and Methods. ""Background.? T lymphocytes induce the transformation of fibroblasts into myofibroblasts, the main mediators of fibrogenesis. The inosine 5��-monophosphate dehydrogenase inhibitor mycophenolate mofetil (MMF) and the anti-CD25 monoclonal antibody daclizumab (DCZ) have been reported to suppress the proliferation of T lymphocytes. Aim.? To evaluate the preventive effects of MMF and DCZ in early stages of bleomycin (BLM)-induced scleroderma. Methods.? This study involved five groups of Balb/c mice (n?=?10 per group).