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Serum IgG to S. aureus,13 Staphylococcus epidermidis and Escherichia coli was determined with ELISA (Navina, Russia). Plasma levels of tumour necrosis factor �� (TNF-��), interleukin-1�� (IL-1��) and interleukin-6 Sotrastaurin solubility dmso (IL-6) were measured by ELISA (Vector-Best Test Systems, Russia), and interferon �� (IFN-��) by ELISA (BioLegend, USA). Serum IgG, IgM and IgA total levels were studied using a turbidometric method (Human Diagnostics, Germany), and IgE by ELISA (R&D, USA). Statistical analysis The sign criterion, Student t test for independent samples, the Mann�CWhitney test, Wilcoxon test, ��2 criterion and regression analysis were used. The Pearson criterion was used if case normal distribution was evident. The Spearman rank order correlation test and multiple regression analysis were used for analysis of association between infections and age. Results Number of days with an infection experienced during a 1-year period The infection results for the 1-year period preceding either the first examination (HC and HR), arthritis onset (eRA) or the last examination (laRA) are presented in figure 1A. There was no significant difference in the median number of infection days between HC (14) and all RA patients together (13). Higher figures were FMO4 noted for HR (53, p as compared with the two earlier time points). Among the 185 RA patients followed from time of diagnosis, a decrease from 56 to 5?days was noted at 8.4?years after diagnosis (pAkt inhibitor We also scored infection types (table 2). There was no infection in 12.4% of HC, 4.0% of HR (p