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Serum insulin was measured by RIA (Pharmacia insulin RIA 100, Pharmacia Diagnostics). The interassay CV was www.selleckchem.com/products/iox1.html (24). The intra- and interassay CV were 4 and 11% respectively. Serum IGFBP1 was also analyzed with an in-house RIA (25). The sensitivity of the RIA was 3?��g/l, and the intra- and interassay CV were 3 and 10% respectively. Quantification of insulin resistance The homeostatic model of insulin resistance (HOMA-IR) was calculated as (fasting serum insulin��fasting blood glucose)/22.5 (26). Statistical analyses Statistical analyses were carried out using STATISTICA Software, version 10 (StatSoft, Tulsa, OH, USA). P values test, are designated as ��. Normality of variables was tested using the Kolmogorov�CSmirnov and Lilliefors tests. Differences between groups in variables that were normally distributed were analyzed using paired and unpaired t-tests, whereas variables that were not normally distributed were analyzed using the Wilcoxon and Mann�CWhitney U tests. Repeated measurements Capmatinib order were studied using repeated measures ANOVA. Body composition has a well-established impact on the regulation of the HPA axis (1). Therefore, correlation analyses were performed between basal cortisol and BMI and waist circumference respectively, using Pearson's correlation coefficient. Multiple linear regressions were performed with selected sets Oxygenase of continuous and categorical variables, as described in the Results section. Variables were included in the multiple regression models if P