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Human microvascular endothelial cell (HMVEC) number in coculture with pericytes expressing constitutively active IKK�� (c.a.), dominant negative IKK�� (d.n.), ... Cytokine secretion from pericyte monocultures and pericyte/HMVEC cocultures To assess signaling molecules that may mediate the proliferative response in endothelial cells, cell culture supernatant was assayed for cytokine concentration. Cell culture supernatants were collected in pericyte/HMVEC cocultures at 24?h after the initiation of coculture and at the corresponding time point in pericyte monocultures. The following cytokines were secreted from pericytes in monoculture in the c.a. IKK�� condition, but were not detected in the d.n. IKK�� condition or e.v. control condition: eotaxin (15.61?��?2.7?��g/mL), granulocyte colony-stimulating INK1197 research buy factor (G-CSF, 17.21?��?4.6?��g/mL), GM-CSF (15.16?��?4.6?��g/mL), fractalkine (CX3CL1, 30.87?��?5.3 ��g/mL), interferon alpha-2 (IFNA2, 8.62?��?1.6?��g/mL), growth-regulated oncogene (GRO, 8.41?��?6.0?��g/mL), (-)-p-Bromotetramisole Oxalate interleukin 6 (IL-6, 45.52?��?9.7?��g/mL), interleukin 7 (IL-7, 11.27?��?2.0?��g/mL), IP-10 (196.56?��?91.3?��g/mL), and macrophage inflammatory protein-1 alpha (MIP-1��, 38.57?��?10.9?��g/mL). There was greater cytokine secretion in the c.a. IKK�� condition compared to both d.n. IKK�� (P?JNJ-26481585 datasheet secretion in the c.a. IKK�� condition compared to both d.n. IKK�� (P?