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Underweight was defined as weight-for-age z-score?Gefitinib research buy weight?=?(z-value?��?S?��?L?+?1)1/L?��?M (de Onis ). The World Health Organization (WHO) Child Growth Standards parameters (L, M and S) were used for the calculation (de Onis ), where ��L�� is the power transformation parameter for normal distribution, ��M�� is the median of weight distribution and ��S�� is the sample standard deviation. The energy requirements for children 6�C8, 9�C11, and 12�C23 months of age were calculated based on the median buy Dasatinib body weight (boys and girls combined) and the FAO estimations for energy requirement (WHO et?al. 2004). The FAO energy requirement estimations for each age group were pooled together. The pooled energy requirements are 79, 80?and 81.3?kcal?kg?1 body weight for 6�C8, 9�C11 and 12�C23-month-old children. Daily energy requirements were calculated by multiplying the energy requirements per kg body weight with median body weight of the population. Then, the AI of EFAs was calculated based on the FAO (2010) recommendations. The median body weights of WHO Growth Standard were 7.93, 8.82 and 10.46?kg for 6�C8, 9�C11 and 12�C23-month-old children, respectively (de Onis ). The associated energy requirements for a reference child are 627, 705 and 851?kcal?day?1 for 6�C8, 9�C11 and 12�C23-month-old children (breastfed and formula fed combined), respectively (Table?2). These results are similar to those recommended by WHO, which are 600, 700 and 900?kcal?day?1 for non-breastfed Oxygenase children (WHO 2005), although the WHO document was published earlier than WHO has released its new growth standard in 2006. The AI for ALA for reference children is equivalent to 0.3�C0.4, 0.3�C0.5 and 0.4�C0.6?g?day?1, and the AI for LA is equivalent to 2.1�C3.1, 2.4�C3.5 and 2.8�C4.3?g?day?1 for reference children aged 6�C8, 9�C11 and 12�C23 months, respectively, based on the calculations using median infant and young child weights from the WHO standard (Tables?3-5) and the FAO (2010) recommended 0.4�C0.6% of energy for ALA and 3.0�C4.5% of energy for LA for infants and young children 6�C23 months of age. This amount is based on the provision of breast milk to meet half of the daily energy needs and the remainder of energy is expected to come from complementary foods (FAO 2010). The AI for DHA ranges from 80 to 125?mg?day?1 for these children (Tables?3-5), using the FAO (2010) requirements for DHA of 10�C12?mg?kg?1 body weight. The 50th percentile body weight decreased from 7.63 to 6.31?kg, from 8.46 to 7.01?kg and from 10.04 to 8.31?kg for 6�C8, 9�C11 and 12�C23-month-old children, respectively, as the prevalence of underweight increased from 5% to 50%.
