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8?��?38?mm needle (Vacuette, Greiner-BioOne, Kremsm��nster, Austria). The tube was then gently homogenized by ten inversions, identified, and stored for a maximum of 4?hours at +4��C in the dark prior to laboratory analyses. Analyses were carried out by the same operator. Before analysis, the tubes were re-homogenized by 20 manual inversions at room temperature. Measurements were performed with a Sysmex KX-21 analyzer (Kobe, Japan) using human settings. Results were excluded if the KX-21 analyzer indicated an analytical error message. As no specific simian control specimens were available, the quality control before each series of analyses was based on the manufacturer's human medium-level control solution (Sysmex Eightcheck-3WP normal level, Sysmex Europe Gmbh, Norderstedt, UMI-77 research buy Germany). The entire panel of analyses obtainable with the KX-21 was carried out: red blood cell count (RBC), hemoglobin concentration (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), RBC distribution width (RDW) expressed as RDW-SD (standard deviation), white blood cell count (WBC), neutrophil, lymphocyte and mixed cells (including eosinophil, basophil, and monocyte) counts, platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW), and platelet large cell ratio (P-LCR) as index of platelet activity. Within-laboratory imprecision and bias were calculated from the results obtained with the human quality control (same as above) tested from January 2009 to April 2011. The histograms of all results were visually inspected. Possible outliers Megestrol Acetate were detected by applying Dixon-Reed's criterion. The reference limits and their 90% CI were determined by nonparametric method. Effects of variation factors were tested using a general linear model with the following factors: age, sex, body weight, origin, and possible interactions of body weight with age see more and origin. Possible differences due to capture vs. purpose-breeding could only be tested in the Tamarinier subgroup. When a significant effect (P?