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13 The cohort will comprise all clinically recognized pregnancies in Denmark starting in 2008 by linking the Danish National Registry of Patients (covering all Danish hospitals), the Danish Fetal Medicine Database, and the Danish Medical Birth Registry.14 Information on exposure to medications will be obtained through linkage with the Danish National Health Service Prescription Database (DNHSPD).15 The cohort from 2008 to 2014 will comprise 589,000 pregnancies with information Forskolin on 424,000 pregnancies resulting in live-born children, ?420,000 undergoing prenatal ultrasound scans, 92,000 terminations, 65,000 miscarriages, 2,000 stillborn children, and 6,000 with other outcomes (including ectopic pregnancies). Data will be updated yearly with information on ?80,000 pregnancies. Danish National Registry of Patients The registry contains information on all in- and outpatient contacts in the hospital system since 1995.16 Diagnoses are coded according to the tenth revision of the International Classification http://www.selleckchem.com/products/cx-5461.html of Diseases. The database includes information on spontaneous and induced abortions and on malformations diagnosed postnatally (ie, also years after birth). Danish Fetal Medicine Database This nationwide clinical quality database was established both to enable quality assessment of the prenatal screening in Denmark and to provide data for research.17 Importantly, it holds information on malformations diagnosed prenatally. All departments involved in prenatal screening (such as nuchal translucency and malformation scans) provide data to this central database. The data are collected in a standardized way as all departments use the same software (Astraia, Munich, Germany) to store the data as part of everyday clinical management. The departments also use identical algorithms to collect and send the data to the central database, hosted by the Danish Regions�� Quality Assessment Unit,18 where they are linked to information from the Medical Birth Registry, the Danish National Registry of Patients, and the Danish Central Cytogenetic Registry using the CPR numbers of the mother and her offspring. The population in the Fetal Medicine Database Dipivefrine consists of women accepting prenatal screening or ultrasound. Data on all first trimester screenings and second trimester malformation scans are included. Between 2008 and 2012, ?92% of the pregnant population in Denmark underwent first trimester screening (eg, 279,105 pregnancies between 2008 and 201217), and ?96% of the pregnancies were scanned between 18 weeks and 22 weeks of gestation. In all,