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It was difficult to establish and implement a standard protocol for obtaining find more the images, as the protocol varied on a case-by-case basis in order to ensure the lowest radiation dose possible for a given patient. In general, variability in the size of the field of view, image resolution, slice thickness, and interval may prevent the collection of standardized images, which potentially would allow a more accurate assessment. In future research, a standard image acquisition protocol should be defined, to the extent possible, in order to ensure an optimally accurate comparison. The ethnic background of each patient was not recorded and was not taken into consideration as an inclusion criterion. Assessing specific ethnic groups may be technically challenging, in light of factors such as migration rates, the increased prevalence of individuals with multiethnic backgrounds, and globalization, which can make it difficult to define appropriate inclusion criteria. Therefore, the individuals analyzed represent only a sample of the population that attended the radiological centers in both countries. However, when our results are compared to those reported in studies analyzing other populations,7,15,16,17,18,20,21,22,23,24,25,26,27,28 it can be concluded that our population samples showed features corresponding to groups of predominantly Caucasian background. The mandibular first molar Enzalutamide generally has one mesial root and one distal root.7 The most frequent variation in the number of roots is the presence of a third root, which is located in the distolingual position and is found with a prevalence >30% in Asian populations.7,15 Histone demethylase In contrast, in the present study, a third root was found in both groups much less frequently (in approximately 3% of the Belgian sample and 6% of the Chilean sample). Other studies, such as that by Curzon et al.20 in an English population or Sch?fer et al.21 in a German population, have reported frequencies of 3.3% and 0.7%, respectively. It is considered normal in Caucasian populations for this anatomical variation to be present with a frequency