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Xijklm FMO5 represents the error because of the smaller scale differences between samples and variability within blocks (anova, SAS institute, 1996). Effects were considered significant if P? of block and the interactive effects of block and other factors were not significant (P?>?0.45), and the effect of block and its interaction with other factors are not presented in order to facilitate data presentation in tables and in text. Least significant difference (LSD) tests were used to separate the levels within the same variable. To quantify the nematode reproduction on different tomato genotypes under two CO2 levels, split-plot was also applied, with CO2 and block as the main effects and tomato genotype as the subplot effect. Proportional data were transformed using the arcsine square root to satisfy assumptions of normality. Data from plant height were square root-ln(X) transformed, and VOC and numbers of galls per gram of root were ln(X?+?10) transformed if necessary. CO2 level, genotype and their interaction had significant effects on plant height selleck kinase inhibitor (Table?A1). All factors, with the exception of the interaction between CO2 level and genotype, as well as the interaction among CO2 level, genotype and nematode, significantly affected plant biomass. Furthermore, all factors and their interactions had significant effects on the foliar TNC?:?N ratio, and with exception of CO2 level, nematode, and interactions among the three factors, all factors and their interactions were significant for root TNC?:?N ratio. In contrast, only genotype and the interaction between genotype and nematode affected the soil C?:?N ratio (Table?A1). Selleckchem PF-06463922 Elevated CO2 increased the height, biomass and the foliar TNC?:?N ratio of all the genotypes (Fig.?1). Regardless of CO2 level, uninfected 35S had highest foliar TNC?:?N ratio among genotypes. Nematode infection reduced the biomass of spr2 plant under ambient CO2 and the biomass of all the genotypes under elevated CO2, but was not significant for plant height under both CO2 levels (Fig.?1). CO2 level, genotype, nematode infection and their interactions (except for CO2?��?nematode) significantly affected the activity of SOD in tomato leaves (Table?A1). Genotype, nematode infection, and their interaction had significant effects on CAT activity in tomato leaves. Furthermore, all factors and their interactions significantly influenced total amino acid and protein contents of leaves (Table?A1). Regardless of CO2 level, uninfected 35S had highest enzyme activities of SOD and CAT among the three genotypes (Fig.?2). Furthermore, elevated CO2 decreased the foliar total amino acid (F1,36?=?325.6, P?
