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In order to test for infants�� visual preferences during the test phase following habituation, a preference score on the percentage of time each infant spent fixating to the novel object (real object or picture) across all three test Nutlin-3 molecular weight trials was calculated by dividing fixation time to the novel object by overall fixation time multiplied by 100. We conducted a 2 �� 2 ANOVA examining the effects of the two age groups (7-month-olds or 9-month-olds) and habituation stimulus format (real object or picture) on the preference score for novel objects. A preliminary ANOVA with a third factor of object identity (mouse, car, frog, or bear) revealed no significant main effect of object identity nor interactions Thymidine kinase with object identity (all Fs 0.11); thus, we collapsed across this factor for the following analyses. The 2 �� 2 ANOVA on the preference score for novel objects with age group and habituation stimulus format as between-participants variables revealed a significant main effect of habituation stimulus format, F(1,69) = 17.38, p 0.36). Infants who were habituated to real objects showed a familiarity preference (M = 46.1%, SE = 1.3), indicating that they kept preferring to look at real objects during the test. For infants who were habituated to pictures of objects, ABT-737 chemical structure our analyses revealed a preference for novel objects (M = 54.8%, SE = 1.6) and therefore, again, for real objects (Figure ?Figure33). In order to contrast the preference scores for novel objects separately for the two habituation stimulus formats against chance level, post hoc single t-tests were performed (Bonferroni corrected). The t-tests confirmed the preference for real objects to be significantly different from chance level for infants who were habituated to real objects, t(40) = -2.93, p