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Studies using film clips were represented in both of these categories, though more studies using film clips found deactivation than activation (for a representative exception, see a paper by the same author (Kreibig et al., 2007) using loss-related film clips). Given the strong theme of loss present in our film clip (parent losing a child) and the anecdotal information that several of our participants used tissues at the conclusion of the sad film clip, we predicted a greater elevation of electrodermal response in the sad film clip measurement than the neutral baseline. Adult attachment Adult attachment was assessed with 13 items from the Relationship Scale Questionnaire (RSQ; Griffin and Bartholomew, 1994; see Supplementary Material for included items). Brennan et al. (1998) suggest that the best fitting model IOX2 for adult attachment involves two dimensions: anxiety, involving fear of separation and an excessive need for approval, and avoidance, involving fear of intimacy and an excessive self-reliance. People scoring high on either (or both) dimension(s) are considered to have insecure attachment, whereas people scoring low on both have secure attachments (Wei et al., 2007). We included nine items relevant to anxious attachment (e.g., ��I worry about being abandoned��) and four items relevant to avoidant attachment (e.g., ��I worry about others getting too close to me��). Items were rated on a 5-point scale, from ��not Transferase inhibitor at all like me�� to ��very like me.4�� Procedure Following consent and psychophysiological setup, all participants viewed a 5 min, 44 s neutral aquatic clip to establish a low-arousal baseline. All participants then rated their mood (Pre-Sad Clip) and viewed a 6 min, Diosmetin 07 s film clip taken from the movie My Sister's Keeper. This clip portrays a mother's last conversation with her dying teenage daughter, and concludes with the girl's death. Participants rated their mood again (Post-Sad Clip), and then were randomly assigned to engage in either a nostalgic or an ordinary event reflection. Following the memory reflection, participants rated their mood a final time (Post-Memory) and then filled out questionnaires assessing demographic information and the adult attachment measure via SurveyMonkey.com. Results Preliminary analyses Did we induce a sad mood state? To determine whether we successfully induced a sad mood state with the film clip, we submitted participants' mood ratings Pre-Sad Clip and Post-Sad Clip to paired samples t-tests. This analysis revealed significant increases in sadness from pre- to post- the sad clip, t(69) = 18.582, p