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Therefore, upon arrival, all patients were Otenabant screened for colonization with MDR bacteria. The following cultures were performed: methicillin-resistant Staphylococcus aureus cultures of nose, throat and perineum in oxacillin-containing enrichment broth, vancomycin-resistant enterococci cultures of perineum in vancomycin-containing enrichment broth; perineum cultures for aminoglycoside-resistant gram-negative bacteria in tobramycin-containing enrichment broth and perineum cultures for extended spectrum ��-lactamase (ESBL) -positive enterobacteriaceae on chromogenic screening agar (ESBL Brilliance; Oxoid, Basingstoke, UK). Where applicable, regular cultures of wounds and catheter-urine were also performed. MDR bacteria see more were defined according to the criteria formulated by the Dutch Working Group for Infection Prevention (WIP) [4]. Patients were hospitalized in a cohort taking hygienic precautions for MDR bacteria. After transfer to a second hospital, cultures were repeated according to the WIP guidelines; isolation measures were lifted only if these were cultures negative. The culture results of all involved hospitals were combined for this study. Patient data and presumed risk factors for carriage of MDR bacteria were extracted from patient records at the Major Incident Hospital. The risk factors were analysed by chi-square test or Fisher's exact test where appropriate; risk factors with a p value click here casualties had been admitted to a Libyan hospital in the preceding 2?months. All patients had received medical care in Libya. Twenty-six patients had been hurt by gunshots, 15 by grenades or missiles, 12 by blunt trauma and three had burn wounds (five patients had injuries with multiple causes). The mean length of stay at the Major Incident Hospital was 1.5?days and 47 of the 51 patients were transferred to their ultimate hospital within 48?h. The remaining four patients stayed at the UMC Utrecht. In all, 32/51 (63%) patients had previously been admitted to a Libyan hospital and 16/51 (31%) had received antibiotic treatment in Libya (excluding perioperative antibiotic prophylaxis). Thirty-four (67%) patients had open wounds, 23 (45%) had cut wounds, 21 (41%) had gunshot wounds, five (10%) had excoriations and three (6%) had burns. Patient characteristics and putative risk factors for colonization with MDR bacteria are summarized in Table?1. Thirty (59%) patients were colonized with MDR bacteria. ESBL-producing enterobacteriaceae were found in 26 (51%) patients, aminoglycoside-resistant enterobacteriaceae in 16 (31%), MDR Acinetobacter spp.