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This difference cannot be explained by the amount of previous experience in the sub-samples, since in either case it was moderate (in sample with negative message M = 2.9, SD = 1.4, and in sample with mixed message M = 2.8, SD = 1.5), and similar in the two cases (t (77) = 0.05 p>0.05, d = 0.06). As well as the effect of the negative and mixed emotional messages on attitude, we also wanted to know: 1) their influence on the probability of performing the risk behavior in the immediate future as estimated by the participants, and 2) the moderating role of experience in the behavior on that relationship. We standardized all the variables to carry out a hierarchical regression analysis on the probability of performing the excessive drinking behavior in the following weeks, with the type of emotional Panobinostat clinical trial message (negative vs. mixed) and experience in carrying out this risk behavior as predicting variables. The main effects were obtained in the first step of the regression and the interaction in the second step, introducing the product between the two independent variables (see Cohen and Cohen, 1983). The results of this analysis revealed a main effect of frequency with which the participants carried out the risk behavior, �� = 0.28, t (75) = 2.58, p but not of the type of emotional message, �� = ?0.19, t (75) = ?1.83, p = 0.07. However, the interaction GUCY1B3 between the type of emotional message and participants�� frequency of performing the risk behavior was significant, �� = ?0.20, t (74) = ?1.92, p Gefitinib of emotional message received. Participants with experience in the risk behavior, on seeing the negative message, indicated that the probability of their continuing to perform the behavior was higher than those who received the mixed message. Moreover, the participants with greater experience who received the mixed message indicated a low probability of repeating the risk behavior, as low as that of participants with little experience of carrying out the behavior.