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- For additional adaptation and testing in vivo plasminogen activators formation of PCI complexes
- For all four clustering strategies, the t-SNE-map resulted in increased substantial cluster enrichment of the various brain areas, and lower Davies-Bouldin scores
- For an additional, tissue-specific comparison, we also extracted DNA from bone samples of five individuals who died in the last 4 years, following the protocol
- For assays of SW480 transduced gene products selected on cobalt agarose (produced and selected as described below)
- For binding assays, sufficient recombinant homodimeric, chimeric proteins containing a siglec domain and a Fc domain per subunit
- For comparison of substrate specificity, an iceLogo software was used to generate substrate specificity logos for amino acids at positions adjacent to the identified cleavage sites
- For detection of AX-B-HSA adducts, blots had been incubated with HRP-streptavidin (one:one thousand dilution), and biotinpositive indicators have been detected with ECL
- For determination of significance of co-localization in microscopy experiments, a multivariate analysis of variance (MANOVA) was performed
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