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SFK SH3, SH2 and kinase area proteins ended up injected in copy above a variety of concentrations at 25uC at a circulation rate of 30 l/min
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SFRP1 concentrations but inhibiting it at higher concentrations with malignant transition in endometrial most cancers
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SKOV-3 cells were pretreated with citrate (500 mM) for 1 hr and MT19c ( mM) or automobile was used for 24 hrs
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Samples have been ready for Western blot analysis in the existence of a protease inhibitor cocktail, which inhibits the activity of the proteasome, calpains and caspases but not that of lysosomal enzymes
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Samples were homogenized and a portion of the supernatant was reserved for protein determination (BCA Assay, Pierce)
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