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- After 24 h incubation, in HepG2 cells, antiproliferation EC50 for Where Scontrol is the percent of supercoiled DNA in the control lane
- After 4 washes, antibody visualization was achieved by the incubation for 3 h at room temperature with Alexa 488-conjugated anti-mouse and/or Alexa 568-conjugated anti-rabbit IgGs
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- After approximately 16 hours of incubation with hormone, cells in each well were lysed using 100L mammalian protein extraction reagent
- After blocking, sections were stained with antibodies or phalloidin and imaged with a Leica DM4000B fluorescent microscope
- After centrifugation, the halo containing PBMCs was carefully transferred into a clean centrifuge tube and washed twice with 7 ml of PBS
- After dedication of airway reactivity, mice ended up sacrificed by cervical dislocation the day soon after final aerosol obstacle
- After eight weeks, palpable tumors were detected only at sites injected with C4-2Luc cells infected with empty vector
- After establishing the whole-cell configuration, membrane capacitance (Cm) was estimated by analyzing the transient charges elicited
- After identifying potential binding sites at HA with the MOE module ``Site Finder'', the test compounds were docked into the 30 cavities with the best PLB
- After incubation at room temperature for 30 min, liquid of each sample well was removed, washed and subsequently incubated with the color reaction TMB Chromogen in the dark for 15 min at room temperature
- After incubation with the appropriate peroxidase-conjugated secondary antibody (DAKO, Denmark), the immunoreactive bands were visualized by chemioluminescence
- After investigating a big number of natural merchandise we confirmed Sargassum fusiforme algae to have HIV inhibiting properties
- After pancreas disgregation, cells were stained with Dithizone to identify the Islets (marked by the arrow) as described in Materials and Methods section
- After prediction, the potential miRNA loci were examined carefully based on the distribution and numbers of small RNAs on the entire precursor regions
- After product molecule is determined and the structureactivity romantic relationship is recognized in silico modeling
- After several failures of in vitro fertilization with ZP-intact oocytes, we performed in vitro fertilization with ZP-free MII oocytes and monitored sperm penetration and PN formation
- After the first 4 weeks, mice in the vector group developed physiological signs of morbidity, such as weight loss, crouching, lethargy and/or disorientation
- After this kind of `counter-variety escape' mutants arise, they can swiftly dominate the mobile population and therefore preclude the enrichment of recombinants in subsequent rounds
- After this time cells were washed with PBS and stimulated with 10% FCS to allow cell recovery for next 18 hrs and later depending on further assays adequately stimulated
- After transfection with survivin siRNA, the expression of these mRNAs were significantly decreased in all cell lines as compared to the untransfected control
- After treatment with wortmannin at 33 mm for 60 min in the transgenic root tip cells, we observed that the PVC marker GFP-RabF2b was induced to form small vacuoles
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- Again, as at eleven times submit-enhance, the vaccine-induced T-mobile responses had been predominantly mediated by CD8 T cells
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- Age and gender do not seem to play distinct role in influencing proliferation rate of degenerative cervical NP cells (data not shown)46105 degenerative cervical NP cell were cultured for four weeks in collagen I scaffold
- Age stratified investigation showed no trends in microalbuminuric or macroalbuminuric individuals (Figure S2)
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