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  20. After 1824 h, the plates were harvested on a FilterMate harvester and analyzed on a 1450 LSC Microbeta TriLux counter
  21. After 24 h in culture, supernatants have been removed and placed on microtiter plates coated with purified anti-IL-2 overnight at 4uC
  22. After 24 h incubation, in HepG2 cells, antiproliferation EC50 for Where Scontrol is the percent of supercoiled DNA in the control lane
  23. After 4 washes, antibody visualization was achieved by the incubation for 3 h at room temperature with Alexa 488-conjugated anti-mouse and/or Alexa 568-conjugated anti-rabbit IgGs
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  39. After approximately 16 hours of incubation with hormone, cells in each well were lysed using 100L mammalian protein extraction reagent
  40. After blocking, sections were stained with antibodies or phalloidin and imaged with a Leica DM4000B fluorescent microscope
  41. After centrifugation, the halo containing PBMCs was carefully transferred into a clean centrifuge tube and washed twice with 7 ml of PBS
  42. After dedication of airway reactivity, mice ended up sacrificed by cervical dislocation the day soon after final aerosol obstacle
  43. After eight weeks, palpable tumors were detected only at sites injected with C4-2Luc cells infected with empty vector
  44. After establishing the whole-cell configuration, membrane capacitance (Cm) was estimated by analyzing the transient charges elicited
  45. After identifying potential binding sites at HA with the MOE module ``Site Finder'', the test compounds were docked into the 30 cavities with the best PLB
  46. After incubation at room temperature for 30 min, liquid of each sample well was removed, washed and subsequently incubated with the color reaction TMB Chromogen in the dark for 15 min at room temperature
  47. After incubation with the appropriate peroxidase-conjugated secondary antibody (DAKO, Denmark), the immunoreactive bands were visualized by chemioluminescence
  48. After investigating a big number of natural merchandise we confirmed Sargassum fusiforme algae to have HIV inhibiting properties
  49. After pancreas disgregation, cells were stained with Dithizone to identify the Islets (marked by the arrow) as described in Materials and Methods section
  50. After prediction, the potential miRNA loci were examined carefully based on the distribution and numbers of small RNAs on the entire precursor regions

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