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- Films with any discordant reading had been go through by a third reader, who was a senior pulmonologist at each of the taking part hospitals
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- Finally, PSA expression on the cell surface can be more locally and rapidly regulated by activity-dependent differential delivery of presynthesized PSACAM to the cell surface
- Finally, membranes were exposed to imaging film (Kodak BioFlexEcono Scientific Supplies, Citrus Heights, CA) and developed using a Kodak X-OMAT 1000A Processor
- Finally, sections were counterstained with haematoxylin (Zymed Laboratory, Cambridge, UK), mounted with VectaMount (Vector Laboratories) and visualized by light microscopy (bright-field microscopy)
- Finally, we used miScript 2 RT kit (from QIAGEN), which applied a various basic principle for reverse transcription of miRNAs
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